Cellular alterations associated with therapy-induced senescence

A large amount of apparently senescent tumor cells are observed after chemo/radio therapy, as indicated by ß-Gal staining in HeLa and MCF7 long-term cultures. The observation of ß-Gal positive cells indicates the coexistence of sub-populations of cells with possible different behaviors in terms of resistance to therapeutic agents. In both camptothecin-treated and...

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Identify therapy-induced senescence

Conventional chemotherapeutics and ionizing radiation can promote therapy-induced senescence in tumor cells in vitro as well as in patients, contributing to or being responsible for tumor recurrence. Here we show...

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Shedding the light on cell biology by fast label-free Raman imaging

Multimodal non-linear optical microscopy allows the study of tumor liver cells in a non-invasive way and in the total absence of exogenous markers, with an acquisition speed compatible with in-vivo applications (2 min for images 300×300 pixels, pixel size 350nm). The combination of Epi-CARS...

Continue Reading Shedding the light on cell biology by fast label-free Raman imaging