Coherent Stokes Raman Scattering imaging is made possible
Within the CRIMSON project, Institut Fresnel CNRS has demonstrated that Coherent Stokes Raman Scattering (CSRS), also known as sCiSsoRS, is a relevant contrast mechanism to image chemical bonds in a variety of biologically relevant samples (see Figure).
CSRS is similar to CARS, but it has been poorly investigated because it was known to be highly contaminated by a 2-photon fluorescent background. The researchers have used a double modulation scheme that has been inspired by the SRS detection scheme to drastically decrease this 2Photon fluorescence background. In the same work, they have demonstrated that CSRS can be phase-matched in the backward (epi) direction. These results open the road for the development of CSRS imaging.